Molecular epidemiology, virology and evolutionary dynamics of human immunodeficiency virus type 1 (HIV-1) circulating recombinant forms

Tee, Kok Keng (2008) Molecular epidemiology, virology and evolutionary dynamics of human immunodeficiency virus type 1 (HIV-1) circulating recombinant forms. PhD thesis, University of Malaya.

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Official URL: http://dspace.fsktm.um.edu.my/handle/1812/477

Abstract

Molecular epidemiological investigation was conducted among various risk populations (n=184) in Kuala Lumpur, Malaysia in 2003-2005, based on the nucleotide sequences of protease and reverse transcriptase regions. In addition to circulating HIV-1 strains, including CRF01_AE (57.1%), subtype B (20.1%) and subtype C (0.5%), we detected a candidate of new circulating recombinant form (CRF). We determined four near full length nucleotide sequences with identical subtype structure from epidemiologically unlinked individuals of different risk and ethnic groups. In this chimera, two short subtype B segments were inserted into the gag-RT region in the backbone of CRF01_AE. The recombinant structure was distinct from previously identified CRF15_01B in Thailand. In agreement with the current HIV omenclature system, this constitutes a novel CRF (CRF33_01B). The overall prevalence of CRF33_01B is 19.0% (35/184). While the prevalence of CRF33_01B is particularly high among injecting drug users (42.0%, 21/50), it is also detected in a substantial proportion of homo/bisexual males (18.8%, 3/16) and heterosexuals (9.8%, 9/92). Moreover, unique recombinant forms comprised of CRF01_AE and subtype B with significant structural relationship with CRF33_01B were detected in 1.6% (3/184) of study subjects, suggesting the ongoing recombination process in Malaysia. This new CRF appears to be bridging viral transmission between different risk populations in this country.

Item Type: Thesis (PhD)
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: MS SITI NUR ATIKAH MOHAMAD RUSDI
Date Deposited: 23 Jul 2013 06:25
Last Modified: 23 Jul 2013 06:25
URI: http://repository.um.edu.my/id/eprint/1017

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