Essential oils and biological activities of three selected wild Alpinia species

Syamsir, Devi Rosmy (2009) Essential oils and biological activities of three selected wild Alpinia species. Masters thesis, University of Malaya.

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Essential oils of three selected wild Alpinia species, namely Alpinia murdochii Ridl., Alpinia pahangensis Ridl. and Alpinia scabra (Blume) Náves, were obtained by hydrodistillation. The chemical components and their composition in the essential oils of the rhizomes and the leaves were investigated using gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and Kovats indices analysis. Some of the components commonly observed in the essential oils of these wild Alpinia species were β- pinene, α-pinene, limonene, γ-selinene, α-terpineol, terpinene-4-ol and sabinene. The chemical components of two closely related species, A. murdochii and A. pahangensis were compared in terms of similarities of compounds. 36.6 % of compounds were similar in their leaf oils and 45 % of compounds were similar in their rhizome oils. The marker compound of Alpinia species, 1, 8-cineole was only present in A. pahangensis rhizome oils and A. scabra leaf oils with low concentrations (0.63 % and 0.08 % respectively). The essential oils obtained were tested for their biological activities namely antimicrobial activity, antioxidant activity and anti-inflammatory activity. For the antimicrobial activity, the minimum inhibitory concentration (MIC) assay was applied. The rhizome oils of these three Alpinia species exhibited potent inhibition against VISA and their MIC values were lower than oxacillin. Meanwhile, A. pahangensis rhizome oils also showed potent activity against Sa 7 (Staphylococcus aureus strain) with a lower MIC value compared to oxacillin.The antioxidant activity was tested using two assays, DPPH free radical scavenging assay and reducing power assay. In DPPH free radical scavenging assay, A. scabra rhizome oil exhibited the highest percentage of inhibition with 55.17 % ± 1.23 at a concentration of 5 mg/ml. At the same concentration, A. scabra rhizome oil also showed the highest reducing power of 1.085 ± 0.004. The assays used for the anti-inflammatory activity were hyaluronidase assay and lipoxygenase assay. In the hyaluronidase assay, at the concentration of 100 μg / μL, all the oils tested showed moderate activity (40.63 ± 4.31 % until 66.38 ± 9.43 %) except for A. pahangensis leaf oils (38.41 ± 6.34 %). Leaf and rhizome oils of A. murdochii and rhizome oil of A. scabra exhibited high inhibition percentages on lipoxygenase assay with 95.37 ± 6.55 %, 91.11 ± 7.82 % and 90.42 ± 0.10 % respectively.

Item Type: Thesis (Masters)
Subjects: Q Science > Q Science (General)
Date Deposited: 31 Jul 2013 03:12
Last Modified: 31 Jul 2013 03:12

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