Expression of p53, pRb, Ki-67 and bcl-2 in Betel-quid induced Oral Mucosal Lesions

RB, Zain (2005) Expression of p53, pRb, Ki-67 and bcl-2 in Betel-quid induced Oral Mucosal Lesions. UNSPECIFIED.

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Abstract

<p align="justify"><font face="Arial"><strong>Kumar SKS, Kipli NP, Zain RB, Choon YF, Peh KB</strong> </font></p> <p align="justify"><font face="Arial"><em>Introduction:</em> Alteration in p53, pRb, Ki-67 and bcl-2 have been implicated in the development of oral cancer although their combined expression in betel quid induced oral mucosal lesions is not clear. The objective of this study is to investigate the expression of p53, pRb, Ki-67, and bcl-2 in oral mucosa of betel quid chewing subjects.<br /> <em>Materials and Methods</em>: A total of 64 tissues sections were obtained from biopsy samples of 50 subjects clinically diagnosed with betel chewer&rsquo;s mucosa (BCM, n=32), BCM with leukoplakia (n=12) and leukoplakia (n=6). Oral squamous cell carcinoma (OSCC, n=10) and normal oral mucosa (n=4) sections were used for comparison. Histopathological analysis was made followed by immunohistochemical detection of p53, pRb, Ki-67, and bcl-2. bcl-2 staining was done in 25 cases of betel chewer&rsquo;s mucosa with or without leukoplakia.<br /> <em>Results</em>: Histopathologic analysis revealed atypical hyperplastic changed in 46% and dysplasia in 4% of cases. 28% cases showed p53 expression in parabasal and superficial layers of epithelium. 14 cases expressed either abnormal pRb or loss of pRb in which 8% of cases had cellular atypia and basal cell hyperplasia. 25 cases (50%) expressed Ki-67 in superficial layers of epithelium indicating high proliferative activity. 36% (9/25) of cases analysed for bcl-2 showed positivity in basal layers of epithelium. Interestingly, 40% (10/16) cases which were bcl-2 negative had high Ki-67 expression.<br /> <em>Conclusion</em>: The findings of our study suggest that alterations of p53, pRb, Ki-67, and bcl-2 occur early in oral mucosal lesions of betel quid chewing patients. This may aid in early diagnosis of potentially malignant oral lesions and oral cancer developing in the betel quid chewing patients who have neither developed clinically recognizable precursor lesion nor exhibit classical dyplastic changes. </font></p> <p align="justify"><font face="Arial">Oral Oncology, MacMillan India; 2005. Vol 10: 148 &ndash; 154.</font></p>

Item Type: Other
Uncontrolled Keywords: oral cancer, immunohistochemistry, chewers mucosa
Subjects: R Medicine > RK Dentistry
Depositing User: MR. ADNAN YAHYA
Date Deposited: 24 May 2008
Last Modified: 15 Jun 2008
URI: http://repository.um.edu.my/id/eprint/61167

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